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Molecular solutions have shown that the vaginal microbiota usually has microbes which were missed in society-centered reports, which includes Lactobacillus inersVerify the pH within your buffer periodically and guarantee optimum pH (seven.eight to 8.0) for DNA extraction. Do not use the buffer out of this pH array. The salt concentration is a vital
This combination is then incubated at 55°C for one hour. Then the sample is phenol-extracted the moment with phenol/chloroform/isoamyl alcohol Option and centrifuged. The aqueous layer is taken out to the contemporary microcentrifuge tube. The DNA is ethanol-precipitated, re-suspended in buffer, and ethanol-precipitated a second time. Following th